In four independent lines, KIN-20/GFP expression was observed throughout larval development and into the adult stage in both hypodermal seam cells and the hermaphrodite-specific neuron (HSN). The spatial pattern of KIN-20::GFP expression overlaps with LIN-42::GFP expression.
In WT animals, miR-48, miR-241, and miR-237 miRNAs were expressed at very low levels during the L1, when LIN-14 is normally expressed, and increased in abundance 20- to 30-fold by the L3 when LIN-14 is down-regulated.
In WT animals, miR-48, miR-241, and miR-237 miRNAs were expressed at very low levels during the L1, when LIN-14 is normally expressed, and increased in abundance 20- to 30-fold by the L3 when LIN-14 is down-regulated.
In WT animals, miR-48, miR-241, and miR-237 miRNAs were expressed at very low levels during the L1, when LIN-14 is normally expressed, and increased in abundance 20- to 30-fold by the L3 when LIN-14 is down-regulated.
In WT animals, LIN-28 is highly expressed upon hatching and during the L1 stage, and expression decreases throughout development, so that by the L3 it reduced 10- to 20-fold.
All forms of LIN-28 were abundant during the L1 stage and decreased to their lowest levels by the L3 stage (3036 h). Titration experiments revealed that the decrease in LIN-28 protein level from 12 to 30 h of larval development was between 10- and 20-fold.
pJW6 contains a 3.4-kb fragment located between 2.0 and 5.4 kb upstream of lin-39 that directs expression in P5.p and P6.p and the syncytial hypodermis, however, expression was observed in few animals (<20%).
mNG::LIN-7, LIN-2::mK2 and mNG::LIN-10 were found to bebroadly expressed in the worm. In L3 larvae, they were prominentlyexpressed in VPCs and neurons, whereas only LIN-7 and LIN-10 weredetectable in the somatic gonad primordium. In the VPCs,endogenous mNG::LIN-7 was strongly cytosolic, frequently found atpunctae, and occasionally localized to basolateral membranes.
mNG::LIN-7, LIN-2::mK2 and mNG::LIN-10 were found to bebroadly expressed in the worm. In L3 larvae, they were prominentlyexpressed in VPCs and neurons, whereas only LIN-7 and LIN-10 weredetectable in the somatic gonad primordium. In the VPCs,endogenous mNG::LIN-7 was strongly cytosolic, frequently found atpunctae, and occasionally localized to basolateral membranes.