In transgenic L1 larvae, fluorescence can be seen in diverse cell types, including hypodermis, muscle, and neurons, but not in the germline. Lineages that are abnormal in
lin-28 mutants, such as lateral and ventral hypodermis, express the
lin-28:GFP fusion. Other cell types, such as head neurons and body muscle also express
lin-28:GFP. The fluorescence is most intense in neurons and occasionally in hypodermal blast cells, and is often weak in or absent from the intestine.