Antibody staining limited to nuclei within pharynx and is detected from beginning of morphogenesis onwards. CEH-22 first detected approx. 330 minutes after fertilization (the lima bean stage) in 11-14 pharyngeal muscle nuclei (probably pharyngeal muscle). At 1.5-fold stage, 14-23 pharyngeal nuclei stain. Cells identified as
m3,
m4,
m5 and
m7. In embryos that have completed elongation (pretzel stage), CEH-22 positive nuclei are identified as
m3,
m4,
m5 and
m7. Also detected in 6 other pharyngeal nuclei, which are believed to be
m1 muscles. After hatching, staining persists in
m1,
m3,
m4,
m5 and
m7 but absent in
m6 and
m2.