In addition to expression described in Expr12377, the two TSP-21::GFP lines generated via the CRISPR-Cas9 system also showed GFP expression in the somatic gonad and vulva in L2-L4 larvae and adults, as well as in the rectal epithelium in L4 larvae. These observations suggest that the enhancer elements for
tsp-21 expression in the somatic gonad, the vulva and the rectal epithelium lie outside of the 10.5kb
tsp-21 genomic region included in pJKL1004.