Uniform expression of
odd-1::GFP was detected in the intestine of embryos and larvae when the transgene was expressed from an extrachromosomal array. To more carefully assess
odd-1::GFP localization, an integrated transgenic line was isolated and embryos from this line double-stained with antibodies specific for GFP and for the intestinal marker MH33. Uniform
odd-1::GFP staining was detected in presumptive gut cells prior to the onset of elongation. However, unlike the more static pattern seen with the unstable extrachromosomal lines, differences in expression in the head and intestine were seen during elongation. The GFP signal increased in the most anterior cells of the intestine, and showed a concomitant relative decrease throughout the rest of the intestine, during the early stages of elongation. As elongation proceeded, additional GFP-positive cells were detected in the head.