Promoter activity of
spp-9 was observed in the entire intestine of animals, strongly in the anterior and posterior ends.
spp-9 transgene expression in the intestine was visible at all developmental stages starting from the first larval stage up to adulthood. We also observed
spp-9 expression in six head neurons. We confirmed the expression of
spp-9 in the AWB and AWC neurons by showing colocalization with a fluorescent marker,
odr-1::RFP (oyIs44), that is specifically expressed in the two AWB and two AWC sensory neurons. The
spp-9 reporter was also visible in a third amphid pair. VisCello for Visualization of Single Cell Data expression data suggests
spp-9 is expressed in the ASH neurons, which is consistent with the observed position of this third neuron pair.