PDK-1/GFP expression is observed in late stage embryos and throughout the life of the animal. In postembryonic animals, PDK-1/GFP expression is observed in the cell bodies and processes of the majority of neurons in the head and tail, in the motor neurons of the ventral nerve cord and neuronal processes along the body of the animal, in the cells and neurons of the pharynx, in intestinal cells, and in hypodermal cells. In L4s and adults, PDK-1/GFP is also expressed in the somatic gonad.
Asymmetric expression of imb-2 in AWCON was also observed in our initial assessment of the imb-2 expression pattern using imb-2 fosmid::GFP in which we fused GFP with the C terminus of IMB-2 by homologous recombination, that rescued imb-2(vy10) mutants.
NOS-2 protein is expressed sequentially during embryogenesis. First detected expression of NOS-2 in the 1-cell stage; at this stage, NOS-2 was present uniformly throughout the embryo. No NOS-2 expression was detected in 2- to 20-cell embryos. NOS-2 expression reappeared in the 28-cell stage in the cytoplasm of the germline blastomere P4. In some embryos, NOS-2 staining appeared to be concentrated in a few perinuclear foci. NOS-2 expression continued in P4 and its two daughters Z2 and Z3 until approximately the 200-cell stage. NOS-2 levels decreased sharply in later stages and were undetectable by the 550-cell stage.