A transcriptional GFP reporter strain MAB336 (hpo- 9p::GFP) was generated. We observed a ubiquitous GFP expression from L1 to adult stage, primarily in the pharynx and head neurons but weaker in the posterior intestine, body wall muscle cells and hypodermal cells. C. elegans hermaphrodites have 8 DAergic neurons, including 4 cephalic neurons (CEPs) and 2 anterior deirid neurons (ADEs) in the head and 2 postdeirid neurons (PDEs) in the posterior. We crossed MAB336 with OH7193, which has mCherry labeled DAergic neurons and found that the GFP signal was co-localized with mCherry labeled DAergic neurons, indicating
hpo-9 was expressed in DAergic neurons.