The expression pattern of endogenously tagged LIN- 46::mCherry differs from the pattern previously reported for a
lin-46 transcriptional reporter transgene (Pepper et al., 2004): We observed LIN-46::mCherry predominately in hypodermal seam cells and in the ventral hypodermal vulval precursor cells (VPCs) at the L3 and L4 stages. Our finding that LIN-46::mCherry expression is restricted to the L3 and L4 stages, combined with a previous report indicating that
lin-46 transcription occurs at all larval stages (Pepper et al., 2004), suggests that
lin-46 is post-transcriptionally regulated.