8 Expression patterns found (0.017 seconds)

Expr15501

Though the tag-63 reporter occasionally reveals faint expression in gonads and intestines, we mostly detected strong expression in neurons such as body, head and tail neurons. In detail, we identified significant expression in sublateral neurons, CANL/ CANR neurons, nerve ring and amphid neurons, ventral cord neurons, tail neurons, and mechanosensory neurons such as ALM. In many of these neurons TAG-63 colocalizes with (neuron-specific kinesin-3) UNC-104 and (synaptic precursor) SNB-1 proteins.

Expr14966

To visualize clathrin at physiological levels, we used CRISPR/Cas9 to generate a clathrin heavy chain reporter construct with an N-terminal GFP tag (GFP::CHC-1), which showed the expected punctate localization pattern in the apical region of hyp7 epidermal syncytium.

Expr1070

SAX-1::GFP was widely expressed in embryos and continued to be expressed in larvae in neurons that contribute axons to the nerve ring, hypodermal cells, including lateral seam cells, and muscle, where the SAX-1::GFP tag displayed a punctate localization.

Expr12811

pbah-1::GFP is expressed in the lateral seam cells, a subset of the hypodermis. Expression was detectable beginning in the early larval stages (L1/L2) and continuing into adulthood, consistent with the temporal expression reported in a functional genomics study. The localization at the protein level was confirmed with a full-length BAH-1 with an added C-terminal HA tag.

Expr8537

When fused to a GFP reporter, the 21-kb region upstream of nsy-7 drove expression in numerous cell types including gut, the amphid sheath glial cells, and head and tail neurons including AWC, ASE, and ASH. In adult animals, nsy-7::GFP was asymmetrically expressed in one AWC neuron. The nsy-7-expressing neuron was identified by crossing a line expressing nsy-7::GFP with a line expressing an srsx-3::mCherry (AWCOFF) reporter or a str-2::dsRed2 (AWCON) reporter. nsy-7 was consistently expressed in the same cell as str-2 (145/158 animals), and contralateral to the cell expressing srsx-3 (99/99 animals). Therefore, nsy-7 appears to be expressed in AWCON but not AWCOFF.

Expr3427

SMU-2::GFP is a nuclear protein. SMU-2::GFP was observed at all stages of development including early embryogenesis and in oocytes. To show that SMU-2 is ubiquitously expressed, embryos and larvae carrying the integrated smu-2::gfp array were costained with the DNA stain DAPI and SMU-2::GFP was observed in essentially every cell that was stained by DAPI. Unlike most transgenes in C. elegans, the smu-2::gfp transgene was expressed in the germ line. To confirm this result with another SMU-2-expressing transgene, a rescuing transgene that placed a HA tag at the very end of the protein were constructed. This construct also led to nuclear expression in all cells, including the germ line.

Expr15691

In L2 and L3 larvae, mNGr::LIN-3 expression was detected in intracellular punctae and on the basal cortex of the AC. No extracellular mNGr::LIN-3 signal could be detected, suggesting that the majority of mNGr::LIN-3 remains attached to the AC surface after its secretion to the plasma membrane, or that a cleavage product of the mNGr::LIN-3 protein is released from the AC and rapidly taken up by the adjacent VPCs or degraded. The same localization pattern was observed with a multicopy GFP::LIN-3 transgene (zhIs67), in which the GFP tag had been inserted at the same position as in the endogenous mNGr::lin-3(zh112) reporter strain. In wild-type larvae, mNGr::LIN-3 was enriched towards the ventral cortex of the AC from the mid-L2 stage until the mid-L3 stage.

Expr15690