The
hlh-14::gfp transgene expresses GFP in the nuclei of several cells in the developing embryo. Prior to morphogenesis, a horseshoe-shaped pattern of cells expresses HLH-14::GFP in the anterior embryo. The expressing cells appear to be neuroblasts but have not been positively identified. During morphogenesis, HLH-14::GFP assumes a complicated, diverse pattern, and no HLH-14::GFP is visible during postembryonic development. In the posterior embryo, HLH-14::GFP was first detect in the bilaterally symmetric blast cells ABplapppa and ABprapppa. A centrally located posterior blast cell, identified as C.aapa, expresses HLH-14::GFP a little later. Around 230 minutes of development, each PVQ/HSN/PHB neuroblast divides to generate an anterior PVQ neuroblast and a posterior HSN/PHB neuroblast. HLH-14::GFP was seen expressed in both of these daughter cells. At 280 minutes of development, the HSN/PHB neuroblast divides to produce a small daughter cell that dies and a larger daughter cell, the HSN/PHB precursor. Both cells contain HLH-14::GFP. Finally, around 310 minutes of development, the PVQ neuroblast divides to produce a posterior daughter cell that dies and the PVQ neuron. Again, HLH-14::GFP was seen in both of these cells.