UNC-69::GFP expression was first detectable in embryos. In older larvae and adults, UNC-69::GFP was expressed in neurons of the anterior, lateral, ventral and retro-vesicular ganglia in the head, and in neurons of the preanal, dorso-rectal and lumbar ganglia in the tail. The fusion protein was also present in the ventral nerve cord (VNC), in the dorsal nerve cord (DNC), in the dorsal and ventral sublateral nerve cords, and in commissural axons. The reporter was expressed in the neurons named CAN, HSN, ALM, PLM, AVM, PVM, BDU, and SDQR, as evidenced by its localization to the cell bodies of these neurons. Expression of
unc-69 in these latter cells was confirmed using an
unc-69::LacZ::NLS fusion. Taken together, these results indicate that
unc-69 is expressed widely, perhaps ubiquitously, in the C. elegans nervous system.