- WBPaper00049942:HeatShock_upregulated_hsf-1(RNAi)
Transcripts that were differentially expressed in different conditions, compared to the hsf-1(+);-HS control, were determined with CuffDiff, which uses the Benjamini-Hochberg correction for multiple testing to obtain the q-value (the FDR-adjusted the p-value).
Transcripts that showed significantly increased expression in hsf-1(RNAi) animals, after heat shock at 33 C for 30min, comparing to in hsf-1(RNAi) animals without heat shock.
- WBPaper00049942:HeatShock_downregulated_hsf-1(RNAi)
Transcripts that were differentially expressed in different conditions, compared to the hsf-1(+);-HS control, were determined with CuffDiff, which uses the Benjamini-Hochberg correction for multiple testing to obtain the q-value (the FDR-adjusted the p-value).
Transcripts that showed significantly decreased expression in hsf-1(RNAi) animals, after heat shock at 33 C for 30min, comparing to in hsf-1(RNAi) animals without heat shock.
- WBPaper00049942:hsf-1(RNAi)_downregulated
Transcripts that were differentially expressed in different conditions, compared to the hsf-1(+);-HS control, were determined with CuffDiff, which uses the Benjamini-Hochberg correction for multiple testing to obtain the q-value (the FDR-adjusted the p-value).
Transcripts that showed significantly decreased expression in hsf-1(RNAi) animals comparing to in control animals, without heat shock.
- WBPaper00049942:hsf-1(RNAi)_upregulated
Transcripts that were differentially expressed in different conditions, compared to the hsf-1(+);-HS control, were determined with CuffDiff, which uses the Benjamini-Hochberg correction for multiple testing to obtain the q-value (the FDR-adjusted the p-value).
Transcripts that showed significantly increased expression in hsf-1(RNAi) animals comparing to in control animals, without heat shock.
- WBPaper00062118:neural-HSF-1_upregulated
DESeq2 v1.22.2, p-value < 0.05 and fold change > 2.
Transcripts that showed significantly increased expression in strain AGD1289 with pan-neuronall expression of HSF-1, comparing to in N2 animals.
- WBPaper00062118:neural-HSF-1_downregulated
DESeq2 v1.22.2, p-value < 0.05 and fold change > 2.
Transcripts that showed significantly decreased expression in strain AGD1289 with pan-neuronall expression of HSF-1, comparing to in N2 animals.
- WBPaper00052959:hsf-1(RNAi)_regulated_miRNA_HS-dependent
The Benjamini-Hochberg correction for multiple testing was then used to determine significantly altered miRNAs between each treatment condition. Micro RNAs with q-value < 0.05 was considered differentially expressed.
MicroRNAs that showed significantly altered expression in hsf-1(+);+HS vs hsf-1(RNAi);+HS. Worms remained on RNAi plates until the L4 larval-stage before being heat shocked by submerging plates in a 33C water bath for 30 minutes.
- WBPaper00066232:hsf-1(RNAi)_upregulated_ash-2
Differential mRNA expression using DESeq2. mRNAs with a FDR < 0.05 and fold change > 2 considered differentially expressed.
Transcripts that showed significantly increased expression in hsf-1(RNAi) comparing to in ash-2 animals injected with vector.