- WBPaper00053020:sta-1(ok587)_upregulated
RNA-seq data were aligned to the C. elegans transcriptome WS190 using bowtie2. Counts were obtained from resulting BAM files using BEDTools, and these were used to generate normalized data tables using DESeq. A cutoff of mean 25 normalized reads (normalized according to DESeq negative binomial distribution) for at least one condition was used, and significantly altered genes were selected (DESeq Benjamini-Hochberg multiple-test correction q < 0.1).
Transcripts that showed significantly increased expression in sta-1(ok587) comparing to in N2 at 4 days post L4 adult hermaphrodite stage.
- WBPaper00053020:sta-1(ok587)_downregulated
RNA-seq data were aligned to the C. elegans transcriptome WS190 using bowtie2. Counts were obtained from resulting BAM files using BEDTools, and these were used to generate normalized data tables using DESeq. A cutoff of mean 25 normalized reads (normalized according to DESeq negative binomial distribution) for at least one condition was used, and significantly altered genes were selected (DESeq Benjamini-Hochberg multiple-test correction q < 0.1).
Transcripts that showed significantly decreased expression in sta-1(ok587) comparing to in N2 at 4 days post L4 adult hermaphrodite stage.
- WBPaper00065945:global-rpoa-2(-)_upregulated_transcript
DESeq2, fold change >= 2, FDR < 0.05.
Transcripts that showed significantly increased expression in global RPOA-2-depleted L1 animals (degron-GFP-rpoa-2;eft-3p-TIR1, +IAA), compared to controls (degron-GFP-rpoa-2,+IAA).
- WBPaper00045217:prg-1_progressively_regulated
Genes with more than 2-fold change in expression level are considered differentially expressed.
Genes altered by more than 2-Fold in late versus early generation prg-1 mutants and prg-1; daf-2 mutants. Samples include prg-1(pk2290), prg-1(n4357), prg-1(tm872), prg-1(pk2290); daf-2(e1368), prg-1(pk2290); daf-2(e1370), prg-1(tm872); daf-2(e1370), prg-1(tm872); daf-2(m41).
- WBPaper00065945:epidermis-rpoa-2(-)_upregulated_protein
DEP, fold change >= 2, p-value < 0.05.
Proteins that showed significantly increased expression in epidermis RPOA-2-depleted L1 animals (degron-GFP-rpoa-2;col-10p-TIR1, +IAA), compared to controls (degron-GFP-rpoa-2,+IAA).