- WBPaper00055981:fog-2(q71)_upregulated
Cuffdiff
Transcripts that showed significantly increased expression in fog-2(q71) comparing to in N2. cDNAs was synthesized using a 3'-anchored oligo-dT primer.
- WBPaper00055981:fog-2(q71)_downregulated
Cuffdiff
Transcripts that showed significantly decreased expression in fog-2(q71) comparing to in N2. cDNAs was synthesized using a 3'-anchored oligo-dT primer.
- WBPaper00040560:hpl-2_embryo_upregulated
Oligos from the tiling array were mapped to chromosome coordinates of the exons from Wormbase WS180. Any oligo that mapped to a gene on both the Watson and Crick strands was excluded. The remaining oligos were then grouped together (perfect match and mismatch) into probe sets and written out into an Affymetrix CDF file. The CDF file was converted into an R-package and loaded into R. The expression values were calculated using the justRMA function from Bioconductor. This used a Benjamini and Hochberg false discovery rate correction.
Transcripts down regulated in hpl-2(tm1489) embryo comparing to N2 in tiling array analysis.
- WBPaper00040560:hpl-2_embryo_downregulated
Oligos from the tiling array were mapped to chromosome coordinates of the exons from Wormbase WS180. Any oligo that mapped to a gene on both the Watson and Crick strands was excluded. The remaining oligos were then grouped together (perfect match and mismatch) into probe sets and written out into an Affymetrix CDF file. The CDF file was converted into an R-package and loaded into R. The expression values were calculated using the justRMA function from Bioconductor. This used a Benjamini and Hochberg false discovery rate correction.
Transcripts down regulated in hpl-2(tm1489) embryo comparing to N2 in tiling array analysis.
- WBPaper00040560:hpl-2_L3_upregulated
Oligos from the tiling array were mapped to chromosome coordinates of the exons from Wormbase WS180. Any oligo that mapped to a gene on both the Watson and Crick strands was excluded. The remaining oligos were then grouped together (perfect match and mismatch) into probe sets and written out into an Affymetrix CDF file. The CDF file was converted into an R-package and loaded into R. The expression values were calculated using the justRMA function from Bioconductor. This used a Benjamini and Hochberg false discovery rate correction.
Transcripts down regulated in hpl-2(tm1489) L3 larva comparing to N2 in tiling array analysis.
- WBPaper00040560:hpl-2_L3_downregulated
Oligos from the tiling array were mapped to chromosome coordinates of the exons from Wormbase WS180. Any oligo that mapped to a gene on both the Watson and Crick strands was excluded. The remaining oligos were then grouped together (perfect match and mismatch) into probe sets and written out into an Affymetrix CDF file. The CDF file was converted into an R-package and loaded into R. The expression values were calculated using the justRMA function from Bioconductor. This used a Benjamini and Hochberg false discovery rate correction.
Transcripts down regulated in hpl-2(tm1489) L3 larva comparing to N2 in tiling array analysis.