- WBPaper00058955:heatshock_upregulated_CE
DESeq2 v 1.18.1, fold change > 2, FDR < 0.01.
Transcripts that showed significantly increased expression in L2 larva stage C. elegans animals after incubated in a 34C water bath for 30min.
- WBPaper00040412:germline-regulated_daf-16_target
All lists used an Fs:Ptab 0.01 cutoff.
Genes that were regulated by DAF-16 in response to germline loss, identified by comparing differentially expression genes between glp-1(e2141ts, 25C) vs. glp-1(e2141ts, 20C) and daf-16;glp-1(e2141ts, 25C) vs. daf-16;glp-1(e2141ts, 20C).
- WBPaper00047070:DAF-16-dependent_UV_response
Differentially expressed genes were determined by ANOVA analysis using the Partek software package.
DAF-16-dependent genes as being significantly induced (FC > 1.5; P < 0.01) following UV treatment in daf-2 mutants and more strongly induced following UV treatment in daf-2 versus daf-2;daf-16, daf-2 versus N2, and N2 versus daf-16.
- WBPaper00035479:daf-16(RNAi)_downregulated
Significance Analysis of Microarray (SAM) was performed to identify genes that were differentially regulated in each of the comparisons.For DAF-16 regulated genes, 1% median false positive discovery rate was used.
Genes upregulated in daf-2(-) compared to daf-16(-); daf-2(-)
- WBPaper00035479:daf-16(RNAi)_upregulated
Significance Analysis of Microarray (SAM) was performed to identify genes that were differentially regulated in each of the comparisons.For DAF-16 regulated genes, 1% median false positive discovery rate was used.
Genes downregulated in daf-2(-) compared to daf-16(-); daf-2(-)
- WBPaper00052959:hsf-1(RNAi)_regulated_miRNA_HS-dependent
The Benjamini-Hochberg correction for multiple testing was then used to determine significantly altered miRNAs between each treatment condition. Micro RNAs with q-value < 0.05 was considered differentially expressed.
MicroRNAs that showed significantly altered expression in hsf-1(+);+HS vs hsf-1(RNAi);+HS. Worms remained on RNAi plates until the L4 larval-stage before being heat shocked by submerging plates in a 33C water bath for 30 minutes.
- WBPaper00065623:16
CellRanger, DecontX, Monocle3, Louvain algorithm.
Single-cell RNA-Seq cell group 16 expressed in: Body wall muscle middle.
- WBPaper00065158:25C_vs_15C_upregulated_AFD
DESeq2, fold change > 2, FDR < 0.05.
Transcripts that showed significantly increased expression in AFD neurons at lin-15(n765ts); oyIs95 animals shifted from 20C to 25C for 16 hours at L4 larva stage, comparing to animals shifted from 20C to 15C for 16 hours at L4 larva stage.