- WBPaper00037055:DAF-16_binding_DNA
DAM methylation profiles derived from three biological replicates fed on daf-2 RNAi (no heat shock) showed a high degree of correlation between replicates. Sequences was identified with methylation peaks in the top 1% of smoothed log2 ratios (log2 ratio > 1.5) and within the gene boundary or 2kb upstream of the translational start. This defined 1135 methylation peaks and 907 associated genes, with a false discovery rate of <5%.
DAF-16 binding DNA target identified by chromatin profiling by DNA adenine methyltransferase identification (DamID).
- WBPaper00045217:prg-1_progressively_regulated
Genes with more than 2-fold change in expression level are considered differentially expressed.
Genes altered by more than 2-Fold in late versus early generation prg-1 mutants and prg-1; daf-2 mutants. Samples include prg-1(pk2290), prg-1(n4357), prg-1(tm872), prg-1(pk2290); daf-2(e1368), prg-1(pk2290); daf-2(e1370), prg-1(tm872); daf-2(e1370), prg-1(tm872); daf-2(m41).
- WBPaper00059609:malt-1_ilc-17.1_nfki-1_downregulated
Cufflinks (v2.2.1). q < 0.05.
Transcripts that showed significantly decreased expression in malt-1(db1194);npr-1(ad609) animals, in npr-1(ad609) ilc-17.1(tm5218) animals, and in npr-1(ad609) nfki-1(db1198) animals, comparing to the wild type control npr-1(ad609).
- WBPaper00059609:malt-1_ilc-17.1_nfki-1_upregulated
Cufflinks (v2.2.1). q < 0.05.
Transcripts that showed significantly increased expression in malt-1(db1194);npr-1(ad609) animals, in npr-1(ad609) ilc-17.1(tm5218) animals, and in npr-1(ad609) nfki-1(db1198) animals, comparing to the wild type control npr-1(ad609).
- WBPaper00047132:skn-1(RNAi)_downregulated_glp-1(ts)-dependent
Fold change > 4.0 for glp-1(ts) regulated genes, and fold change < 0.67 for skn-1(RNAi) regulated genes.
Genes with < 0.67 fold decreased expression in skn-1(RNAi) animals comparing to in N2 control at 25 centigrade. Fold change = glp-1(ts) + skn-1 RNAi vs. glp-1(ts) + vector
- WBPaper00050094:glp-1(gf)_downregulated
Fold change > 2.
Transcripts that showed significantly decreased expression in the dissected gonads of gld-2(q492) gld-1(q485) (I); glp-1(ar202)(III) (glp-1(gf) NOTCH ON), comparing to in the dissected gonads of gld-2(q492) gld-1(q485) (I); glp-1(e2144) (glp-1(lf) NOTCH OFF)