- WBPaper00035227:bio-electrospray_regulated
Differences between treatments were visualized by principal component analysis (PCA) plotting with MeV/TM4. Data were initially filtered out for missing values and then subjected to a CLEAR test that combines differential expression and variability using the GEPAS web server at http://www.gepas.org. In our case, the false discovery rate was set to a stringent level of 5 per cent.
Genes regulated by bio-electrospray.
- WBPaper00035227:heat_shock_regulated
Differences between treatments were visualized by principal component analysis (PCA) plotting with MeV/TM4. Data were initially filtered out for missing values and then subjected to a CLEAR test that combines differential expression and variability using the GEPAS web server at http://www.gepas.org. In our case, the false discovery rate was set to a stringent level of 5 per cent.
Genes regulated by heat shock.
- WBPaper00055013:HMG-4_interacting_protein
N.A.
Proteins that interact with HMG-4-HA in BAT1967 [hmg-4(bar32[hmg-4-3xHA]) III] at L4 larva stage according to Co-Immunoprecipitation with subsequent Mass Spectrometry analysis.
- WBPaper00046217:mev-1(kn1)_upregulated
The error threshold for peptide spectrum matches was set to a 1% false discovery rate (FDR). Protein expression was measured by the total of identified spectral counts normalized by the total number of spectral counts in each MS run in order to adjust for the variation in signal across runs.
Proteins that showed increased expression in mev-1(kn1) comparing to in N2, according to liquid chromatography and mass spectrometry analysis.
- WBPaper00046217:mev-1(kn1)_downregulated
The error threshold for peptide spectrum matches was set to a 1% false discovery rate (FDR). Protein expression was measured by the total of identified spectral counts normalized by the total number of spectral counts in each MS run in order to adjust for the variation in signal across runs.
Proteins that showed decreased expression in mev-1(kn1) comparing to in N2, according to liquid chromatography and mass spectrometry analysis.
- WBPaper00060278:atp-2(RNAi)_upregulated
Cufflinks 2.2.1
Transcripts that showed significantly increased expression in glp-4(bn2);atp-2(RNAi), comparing to in glp-4(bn2) animals injected with empty vector.
- WBPaper00040135:heat-shock_upregulated
Proteins with more than 4 fold change in expression level are considered differentially expressed.
Proteins that showed more than 4 fold of expression increase after heat shock.