- WBPaper00040858:eQTL_regulated_old
Authors permuted transcript values and used a genome-wide threshold of log10 P-value = 2, which resembles a false discovery rate (FDR) of 0.0136.
Genes with expression level regulated by genotype (N2 vs CB4856) at Old adults stage (214 hours at 24 centigrade).
- WBPaper00061527:R10H10.3-T21H3.1
All available microarray datasets for the GPL200 platform (Affymetrix C. elegans Genome Array) were obtained from the GEO repository. This included 2243 individual microarray experiments. These were normalized against each other with the software RMAexpress (Bolstad, 2014). Based on these normalized values, Pearsons correlation coefficients were obtained for each probe-probe pair of the 22,620 probes represented on this array type. The resulting list of correlation coefficients was then ranked to generate the ranked coexpression database with information on each probe represented on the GPL200 platform.
Coexpression clique No. 214, R10H10.3-T21H3.1, on the genome-wide coexpression clique map for the nematode GPL200 platform.
- WBPaper00028802:intestine_unique
To select a set of genes that are candidates to be expressed strongly and exclusively in the intestine, authors consider only genes for which the I/S tag ratio >= 3 and for which the tag number in the intestine library is >= 50. One hundred genes meet these two criteria. Twenty of these 100 genes encode ribosomal proteins or are involved in ribosome assembly, suggesting that the intestine persists as the major site of ribosome synthesis in the adult worm. However, because most ribosomal protein genes are unique in the genome and therefore must be widely expressed at other developmental stages, these genes were removed from the list to leave the set of 80 highly-expressed intestine-specific or intestine-enriched (non-ribosomal) genes.
Genes with unique expression in intestine, according to SAGE analysis on dissected intestine.
- WBPaper00040858:eQTL_age_regulated_aging
For model 2, authors used 100 permutations to estimate the FDR threshold. Per permutation, genotypes and ages were independently randomly distributed, keeping the among-gene structure intact. Then for each spot (23,232) on the array, model 2 was tested. The obtained P-values were used to estimate a threshold for each of the explanatory factors. Authors also used a genome-wide threshold of -log10 P-value = 2, which resembles an FDR of 0.072 and 0.060 for marker and the interaction age-marker for the developing worms and FDR of 0.050 and 0.065 for marker and age-marker for the aging worms. For the physiological age effect, authors used a log10 P-value = 8 in developing worms (0.012 FDR) and -log10 P-value = 6 (0.032 FDR).
Genes with expression level regulated by genotype (N2 vs CB4856) and age at old adults stage (214 hours at 24 centigrade).
- WBPaper00040858:eQTL_regulated_aging
For model 2, authors used 100 permutations to estimate the FDR threshold. Per permutation, genotypes and ages were independently randomly distributed, keeping the among-gene structure intact. Then for each spot (23,232) on the array, model 2 was tested. The obtained P-values were used to estimate a threshold for each of the explanatory factors. Authors also used a genome-wide threshold of -log10 P-value = 2, which resembles an FDR of 0.072 and 0.060 for marker and the interaction age-marker for the developing worms and FDR of 0.050 and 0.065 for marker and age-marker for the aging worms. For the physiological age effect, authors used a log10 P-value = 8 in developing worms (0.012 FDR) and -log10 P-value = 6 (0.032 FDR).
Genes with expression level regulated by genotype (N2 vs CB4856) at old adults stage (214 hours at 24 centigrade).
- WBPaper00062056:lin-37(n758)_upregulated
A gene model was built based on the WS260 annotation. Tag counts for each gene were extracted from STAR aligned BAM files, anddifferential gene expression between N2 and mutant backgrounds was tested using DESeq2. A false discovery rate(FDR) < 0.01 and LFC > 0.5849 was used to define genes as upregulated, and FDR < 0.01 and LFC < -1 was used to define genes asdownregulated.
Transcripts that showed significantly increased expression in lin-37(n758) comparing to in N2 animals at starved L1 larva stage.
- WBPaper00062056:dpl-1(n2994)_downregulated
A gene model was built based on the WS260 annotation. Tag counts for each gene were extracted from STAR aligned BAM files, anddifferential gene expression between N2 and mutant backgrounds was tested using DESeq2. A false discovery rate(FDR) < 0.01 and LFC > 0.5849 was used to define genes as upregulated, and FDR < 0.01 and LFC < -1 was used to define genes asdownregulated.
Transcripts that showed significantly decreased expression in dpl-1(n2994) comparing to in N2 animals at starved L1 larva stage.
- WBPaper00062056:efl-1(se1)_downregulated
A gene model was built based on the WS260 annotation. Tag counts for each gene were extracted from STAR aligned BAM files, anddifferential gene expression between N2 and mutant backgrounds was tested using DESeq2. A false discovery rate(FDR) < 0.01 and LFC > 0.5849 was used to define genes as upregulated, and FDR < 0.01 and LFC < -1 was used to define genes asdownregulated.
Transcripts that showed significantly decreased expression in efl-1(se1) comparing to in N2 animals at starved L1 larva stage.
- WBPaper00062056:lin-35(n745)_downregulated
A gene model was built based on the WS260 annotation. Tag counts for each gene were extracted from STAR aligned BAM files, anddifferential gene expression between N2 and mutant backgrounds was tested using DESeq2. A false discovery rate(FDR) < 0.01 and LFC > 0.5849 was used to define genes as upregulated, and FDR < 0.01 and LFC < -1 was used to define genes asdownregulated.
Transcripts that showed significantly decreased expression in lin-35(n745) comparing to in N2 animals at starved L1 larva stage.
- WBPaper00062056:lin-36(we31)_downregulated
A gene model was built based on the WS260 annotation. Tag counts for each gene were extracted from STAR aligned BAM files, anddifferential gene expression between N2 and mutant backgrounds was tested using DESeq2. A false discovery rate(FDR) < 0.01 and LFC > 0.5849 was used to define genes as upregulated, and FDR < 0.01 and LFC < -1 was used to define genes asdownregulated.
Transcripts that showed significantly decreased expression in lin-36(we31) comparing to in N2 animals at starved L1 larva stage.