To identify WAGO-1 protein interactors, authors immunoprecipitated FLAG-tagged WAGO-1 and analyzed the coprecipitated proteins by SDS-PAGE. This analysis identified a 100 kDa protein that coprecipitated with FLAG::WAGO-1 (later called
rde-12 in this paper). Tandem mass spectrometry unambiguously identified this protein as F58G11.2, which is predicted to encode a DEAD box RNA-binding ATPase most similar to Vasa/DDX4 and Belle/DDX3.