After aldicarb treatment, ACR-16::GFP puncta fluorescence was significantly increased in rig-3 mutants (35%, p < 0.001), while levels in wild- type animals were unaltered.
In wild-type animals, the ACR-16::GFP fusion proteinwas strongly accumulated at the tips of the muscle arms and in punctated clusters along the nerve cords. However, in the eat-6(ad467) and eat-6(ad601) mutants, more GFP puncta were mislocalized to the trunks of the muscle arms compared to the wild type.