In
unc-104 mutants, both VAChT and monoclonal ChAT immunoreactivities were more concentrated in the somas of a subset of neuronal cell bodies. However, although VAChT immunoreactivity was significantly decreased in the dorsal nerve cord and nerve ring with any of the anti-VAChT antibodies, anti-ChAT staining was still present in the nerve ring with the smooth (R388) and mixed (MAbs1401/1415/1432) antibodies.