"Consistent with the yeast two-hybrid results , we detected GFP : : SNX-1 in IPs of CUP-2 : : 3xFLAG , indicating that CUP-2 and SNX-1 physically associate ( Figure 1C ) ."
cup-2(ar506) (or cup-2 RNAi) results in increased levels of GFP::MCA-3 at the plasma membrane as measured by quantitative microscopy. Western blot analysis of immunoprecipitated GFP::MCA-3 indicated that the increased GFP::MCA-3 signal is due to increased levels of GFP::MCA-3 protein in cup-2 mutant worms.
"The size and approximate number of endosomes labeled with RME-8::GFP in cup-5(ar465) coelomocytes is similar to those in cup-5(+), indicating that these endosomal compartments are relatively normal (Fig. 5)."