"b-naphthoflavone, which in mammals induces CYP1A, showed the highest potential to induce C. elegans CYP genes. In particular CYP35C and 35A were induced (Fig. 2B). A more modest induction of CYP gene expression was assessed for subfamilies 35B, 31A, and 34A, respectively."
This RNAi enhanced the tau-induced uncoordinated (Unc) phenotype (caused by the expression of mutant human tau -FTDP-17) as well as the Unc phenotype some/all of the four Unc lines tested (unc-3, unc-76, unc-78 and unc-87).
The expression of cyp-35a3, cyp-35a5 and cyp-35c1 was greatly increased by up to 100-fold after 1.5 hr TBZ exposure in gfp controls compared to those exposed to DMSO.
The expression of cyp-35a3, cyp-35a5 and cyp-35c1 was greatly increased by up to 100-fold after 1.5 hr TBZ exposure in gfp controls compared to those exposed to DMSO.
Diminish the reproduction decline caused by xenobiotics (PCB52, fluoranthene, lansoprazole, or atrazine) when RNAi against cyp-35A1,A3,A5 and C1 is done in the cyp-35A2(gk317) cyp-35A4(ok1393) background.
The expression of cyp-35a3, cyp-35a5 and cyp-35c1 was greatly increased by up to 100-fold after 1.5 hr TBZ exposure in gfp controls compared to those exposed to DMSO.
"To confirm their expression changes from the RNA-seq experiments, we designed quantitative real-time PCR primers and analyzed their expression from independent C. elegans ethanol-exposed samples... The most highly increased cyp gene according to RNAseq cyp-33B1 was also the most highly increased when compared using quantitative real-time PCR(qRT-PCR). cyp-35B2, cyp-13B1, and cyp-35B1 also displayed high upregulation in RNA-seq and qRT-PCR experiments."
In daf-2(e1370) adults, cyp- 35B1:GFP expression was substantially reduced by hsf-1 RNAi, and cyp-35B1 mRNA was significantly reduced by hsf-1 RNAi as well.