When three E3 ubiquitin ligases (marc-6, hrd-1/sel-11, and hrdl-1) were simultaneously perturbed by mutation or RNAi, worms exhibited a significant synthetic slow growth phenotype.
The ire-1(zc14) mutation completely suppressed the induction of expression of the hsp-4::GFP transgene resulting from RNAi of ufd-1, hrd-1, or cdc-48.1/cdc-48.2, implicating ire-1 in the regulation of hsp-4 expression