Although a low-intensity green CED-4 signal was detected in nuclear membranes of unirradiated germcells, the large majority of CED-4 was present in mitochondria before irradiation. At 36 hours postradiation, the CED-4 signal was markedly reduced in mitochondria, relocalizing primarily to nuclear membranes as bright green platform like structures. In eight worms, overall reduction in CED-4 mitochondrial colocalization upon irradiation was ~50% (P < 0.0001), abrogated in
lagr-1(
gk327);opls219. Consistent with the anti-CED-4 antibody staining the loss of mitochondrial CED-4 signal in opIs219 was accompanied by a twofold increase in nuclear CED-4 signal, blocked entirely in
lagr-1(
gk327);opls219 (to 0.9 +/- 0.1 fold of control).