"In order to verify the gene regulation, we performed qRT-PCR on the 13 genes from ABU and LPR gene families using four independent MeHg-treated and control samples from L4 animals (Fig. 2). The 6 LPR family members were confirmed to be up regulated and all 7 ABU family members were confirmed to be down-regulated significantly... The lpr genes had more modest fold changes in qRT-PCR than in RNAseq. The most highly up-regulated lpr gene,
lpr-4, according to RNA-seq (31-fold) was only 2-fold up-regulated in qRT-PCR."