Genes directly involved in synaptic transmission and calcium sensing at neuromuscular junctions, such as
unc-25/ glutamic acid decarboxylase,
snt-1/synaptotagmin,
unc-13/ Munc13, and
unc-10/RIM1, all had statistically increased expression in
wdr-23 mutants. qRT-PCR of
unc-25/ glutamic acid decarboxylase and
snt-1/synaptotagmin (but not the negative control
unc-17/VAChT) verified a roughly three-fold increase in expression in
wdr-23 mutants, in agreement with the RNA sequencing results.