"To quantify the synaptic decrease of the L-AChRs in
emc-6(
kr150), we generated a knock-in strain carrying the
unc-29 L-AChR subunit endogenously tagged with tagRFP using the MosTIC technique (46). Consistent with immunostaining of LAChRs, we observed an 82% decrease of the amount of L-AChR at the NMJ (Fig. 3 D and E). The remaining L-AChRs still formed synaptic clusters in
emc-6(
kr150) mutants."