"100 RNAi clones which restored ckb-2p::gfp activation in the cdc48.2(-/-) mutant background specifically under tunicamycin treatment were identified as ER-stress-dependent suppressors of cdc48.2(-/-) (Fig 1E, Supplementary Table S4)."
37 mutant worm strains were screened with an RNAi library consisting of 1,860 bacterial feeding strains targeting 1,744 different worm genes. All of the RNAi clones that gave a stronger RNAi phenotype in at least one worm strain compared with wild-type worms were retested in all of the 37 query strains (in duplicate) in order to reduce the rate of false negatives. All interactions from this second round of screening were retested, and interactions observed in at least two of four repeats were considered as positives, giving a total of 377 interactions between query strains and library RNAi clones (equivalent to 349 interactions between 162 genes)