"we performed transcriptome analysis on a selected panel of PMK-1 and DAF- 16-regulated genes using quantitative real-time PCR. Six of the eight PMK-1-regulated genes were downregulated (~2-5-fold) following 48 hours infection by S. aureus of worms that were not treated with UE-12 (Fig. 5A). On the other hand, in infected worms treated with 200 mg/mL UE-12, five of these 6 genes -
clec-67, C17H12.8,
dod-24, F35E12.5 and F55G11.8 - demonstrated a 2.8-4-fold induction in expression upon supplementation with UE-12 (p<0.05), implying that UE-12 promoted the host antimicrobial response. UE-12 also significantly reduced the suppression of T24B8.5 from ~5-fold to ~2-fold (p<0.05) (Fig. 5A)."