A bacterially produced, affinity-purified CES-1 fusion protein consisting of the C-terminal half of CES- 1 (which includes all five zinc fingers of CES-1) fused to glutathione S-transferase (GST) could bind and shift a radioactively labeled 390 bp DNA fragment consisting of wild- type Region B, including the four Snail-binding sites.
Sequence inspection revealed that Region B contains four closely spaced Snail- binding sites, DNA-binding sites for members of the Snail family of zinc-finger transcription factors (Hemavathy et al., 2000). The core motif of three of these binding sites is completely conserved in C. briggsae (binding sites I, II and IV; 6/6 bases identical), and one of them has one base change in C. briggsae (binding site III; 5/6 bases identical). The sequence of binding sites I and II of C. elegans is a perfect match to the sequence of the core motif of a consensus Snail-binding site (5'-CACCTG-3') whereas the sequences of binding sites III and IV have one mismatch to the consensus sequence (5'- CATCTG-3' and 5'-CAGCTG-3', respectively). Binding of CES-1 to Region B was severely reduced after the introduction of point mutations that destroy the core motif of the four Snail-binding sites.
"ENDU-2 interacts with RNA polymerase II to activate Pol II after HS."; "Co-immunoprecipitation of ENDU-2::EGFP with AMA-1 (Pol II subunit A) is RNA-dependent."; "ENDU-2::EGFP but not ENDU-2(E454Q)::EGFP or ENDU-2(E460Q)::EGFP is co-immunoprecipitated with AMA-1"
"ENDU-2 interacts with RNA polymerase II to activate Pol II after HS."; "Co-immunoprecipitation of ENDU-2::EGFP with AMA-1 (Pol II subunit A) is RNA-dependent."; "ENDU-2::EGFP but not ENDU-2(E454Q)::EGFP or ENDU-2(E460Q)::EGFP is co-immunoprecipitated with AMA-1"