"Larvae were fixed and stained with rabbit anti-GFP, rabbit anti-vinculin/ DEB-1 (R3-3), and either MH4 (cIFs), MH5, or MH46 (anti-myotactin). Animals negative for GFP and with wildtype distribution of vinculin are nonrescued animals whose muscle cells are attached. In these animals, the distribution of cIFs, the MH5 protein, and myotactin is similar to that of wild-type worms (data not shown), as is the distribution of MH5 protein and myotactin in regions of animals with detached muscle where hypodermis still contacts muscle (Fig. 3D and E, arrows), demonstrating that initial MH5 protein, myotactin, and cIF localization is not
mua-6-dependent. At the sites of muscle displacement, myotactin, which localizes to the hypodermal membrane adjacent to muscle, colocalizes with the muscle cells (Fig. 3C, D, and I, arrowheads). The MH5 protein, which localizes to the hypodermal membrane adjacent to muscle and that adjacent to cuticle, colocalizes with both the displaced muscle and the bodywall where muscle has detached (Fig. 3E, F, and J, arrowheads). This is also true for MH4-positive cIFs (Fig. 3G, H, and K)."