In wild type, lin-32p::mRFP::unc-60A 3'UTR was intensely detected in every R(n).a + p. However, in plx-1 mutants, R(1).a + p, R(2).a + p and R(5).a + units displayed pronounced decreases in expression of mRFP.
In wild type, lin-32p::mRFP::unc-60A 3'UTR was intensely detected in every R(n).a + p. However, in smp-1smp-2 mutants, R(1).a + p, R(2).a + p and R(5).a + units displayed pronounced decreases in expression of mRFP.
"ectopic expression of the MYO-3 muscular marker expression (Miller et al., 1983) was followed in early and late adult tumors (Figure S5A). Cytoplasmic expression of MYO-3, which is distinct from the signal generally observed in sheath cells around wild-type gonads, was detected in PGL-1-negative cells in a small amount of old adult tumorous germlines (Figure S5A). These cells formed distinct "clonal" groups (Figure S5A), substantiating our previous finding that cells kept dividing after losing P granules (Figure 3)."
Expression of mec-2-lacZ in the ALML/R, AVM, and PVM touch cells was greatly decreased (<5% of the cells stained); 67% of the PLML/R cells still stained in mec-3(u6).