37 mutant worm strains were screened with an RNAi library consisting of 1,860 bacterial feeding strains targeting 1,744 different worm genes. All of the RNAi clones that gave a stronger RNAi phenotype in at least one worm strain compared with wild-type worms were retested in all of the 37 query strains (in duplicate) in order to reduce the rate of false negatives. All interactions from this second round of screening were retested, and interactions observed in at least two of four repeats were considered as positives, giving a total of 377 interactions between query strains and library RNAi clones (equivalent to 349 interactions between 162 genes)
37 mutant worm strains were screened with an RNAi library consisting of 1,860 bacterial feeding strains targeting 1,744 different worm genes. All of the RNAi clones that gave a stronger RNAi phenotype in at least one worm strain compared with wild-type worms were retested in all of the 37 query strains (in duplicate) in order to reduce the rate of false negatives. All interactions from this second round of screening were retested, and interactions observed in at least two of four repeats were considered as positives, giving a total of 377 interactions between query strains and library RNAi clones (equivalent to 349 interactions between 162 genes)
double RNAi in all combinations (ptr-1/ptr-6/ptr-10) did not show interaction, only when RNAi of all three is done is the enhancement interaction observed
Most vab-3(ns157) animals lacked hlh-17::GFP and ptr- 10::myrRFP expression in dorsal CEPsh glia, whereas nearly half expressed these reporters in ventral CEPsh glia.