SNB-1::VENUS was still mislocalized in the non-presynaptic region of RIA in the ppk-1 overexpression strain with ttx-7(nj50) mutation, as observed in ttx-7(nj50) single mutant animals.
SER-1 axonal localization was severely defective in animals carrying a null mutation in the APB-3/AP-3 b subunit. In apb-3/AP-3b mutants, SER-1 was diffusely localized to both the axonal and dendritic segments of RIA.
The RIA-specific expression of egl-8 cDNA in ttx-7;egl-8 double mutants substantially reduced the fraction of normal isothermal tracking behavior, and also disrupted localizations of SNB-1 and SYD-2 similarly to ttx-7 single mutants