Addition of the RIM C2A domain to the PDZ domain of RIM (GFP-PDZC2ARIM), but not the C2A domain of RIM alone (GFP-C2ARIM), was sufficient to localize RIM and restore ELKS localization. Interestingly, the localization of GFP-PDZC2ARIM is dependent on ELKS expression.
"UNC-13L C2A domain tightly binds the UNC-10/RIM ZF domain, and that this interaction was eliminated by a double mutation in the ZF domain (K77/79E), which is analogous to mutations that disrupt binding of the corresponding mammalian Munc13-1 and RIM domains"
"UNC-13L C2A domain tightly binds the UNC-10/RIM ZF domain, and that this interaction was eliminated by a double mutation in the ZF domain (K77/79E), which is analogous to mutations that disrupt binding of the corresponding mammalian Munc13-1 and RIM domains"
"Caenorhabditis elegans ELKS is an active zone protein that directly interacts with the postsynaptic density-25/Discs large/zona occludens (PDZ) domain of RIM."
Antibody specific to Nup35 gave rise to clear nuclear rim staining in control embryos, Nup35 was completely absent from the chromatin of npp-8 RNAi embryos.
In unc-104(e1265) animals, although synaptic vesicle proteins and synaptic vesicles accumulate in neuronal cell bodies11, Rim was still concentrated in puncta in the nerve cord.
Antibody specific to Nup96 gave rise to clear nuclear rim staining in control embryos, the nuclear accumulation of Nup96 was diminished and cytoplasmic foci were observed in npp-8 RNAi embryos.
Antibody specific to Nup153 gave rise to clear nuclear rim staining in control embryos. However, in Nup155 RNAi embryos, Nup153 still associated with chromatin but no longer acccumulated at the nuclear periphery.