"Owing to the reduction and disorganization of DYN-1-GFP foci at the cortex, we were interested in determining whether
arx-2 RNAi led to a disruption in clathrin levels. We generated cortical Z-series projections of wild-type or control embryos expressing GFP-CHC-1 (Sato et_al, 2005). In these embryos, there appeared to be little, if any, asymmetry in the localization of GFP-labeled foci (Supplemental Figure S3). In embryos depleted of ARX-2, however, there is a strong accumulation of GFP-labeled puncta at the anterior cortex (Supplemental Figure S3). We also observe more "open" puncta in
arx-2 RNAi-treated embryos than in wild-type or control embryos expressing this construct."