Interestingly, twk-1(tn1397);acy-4(ok1806) animals do not exhibit an adult-onset Unc phenotype. twk-1 mutant alleles are recessive suppressors of acy-4(lf) sterility, and acy-4(ok1806) is a recessive suppressor of the twk-1(tn1397) movement defect.
Compared with the clec-38(+) animals, 80% of mutants heterozygous for the clec-38 mutation have severe morphological defects and 100% of mutants homozygous for theclec-38 mutation die as embryos.
twk-36::GFP express in the excretory cell of control animals. In contrast, there is a consistent and complete loss of GFP expression in the excretory cell of ceh-6(RNAi) treated animals for twk-36::GFP.