As determined by expression of Pdel-1gfp, which is expressed specifically by VB motor neurons, two-thirds of the VB motor neurons of unc-3 mutants also failed to develop normally.
As assessed using the Pacr-5gfp reporter, which is expressed in DB and VB motor neurons, the number of embryonic DB motor neurons of L1 unc-3 mutants was reduced from 5.4 0.1 in the wild type to 1.5 0.1 in unc-3 mutants.
During the mid-L2 larval stage, del-1::GFP expression in the ventral nerve cord is largely restricted to the VB class of motor neurons. In the unc-37 mutant, del-1::GFP is also expressed ectopically in the VA motor neurons in mid-L2 larvae.