IDA-1::GFP levels in
pag-3(
gv560) mutant animals is markedly increased in VC4, VC5 and ALA neurons and also is observed in four additional neurons (P9-P12 descendants) in the posterior region. Transgenic IDA-1::GFP and endogenous IDA-1 levels in
gv560 mutants are elevated 5-fold and 8-fold, respectively, in
pag-3(
gv650) mutants compared to wild type animals as determined by Western blot analysis.