At 20C,
fog-3 mRNA was increased 17.6-fold in
wdr-5.1;wdr- 5.2 double mutants compared to that of WT. No changes were found in single
wdr-5.1(
ok1417),
wdr-5.2(
ok1444), or
set-2(
tm1630) mutants, again discon- necting the defects in
fog-3 regulation and H3K4 methylation, since H3K4 methylation defects are fully penetrant at 20C in both of these mutants. At 25C, however,
fog-3 mRNA levels increased dramatically in both the wdr- 5.1 single mutant and
wdr-5.1;
wdr-5.2 double mutant by 48- and 77-fold, respectively.