"To experimentally validate whether loss of NTH-1 and XPA-1 induces oxidative stress, we took advantage of the established reporter strain CL2166, which expresses green fluorescent protein (GFP) under the control of the glutathione-S-transferase GST-4 promoter [32]... Depletion of NTH-1 or XPA-1 by RNAi significantly increased the number of foci to 26 and 25, respectively (p < 0.001) (Figure 7), thus demonstrating that even transient depletion of NTH-1 or XPA-1 induces oxidative stress responses... The
gst-4::GFP reporter assay therefore experimentally validated the high throughput genomic results and confirmed that loss of NTH-1 or XPA-1, but not both, leads to oxidative stress and activation of oxidative stress responses."