"KNL-1 still localized to kinetochores in embryos depleted of either ceNDC-80 or HIM-10 (Fig. 5B). However, the level of kinetochore-localized KNL-1 was significantly reduced relative to identically processed control embryos (Fig. 5B)... KNL-1 was reduced to ~50% of wild-type levels in depletions of either CeNDC-80 or HIM-10, suggesting that reduction of KNL-1 protein levels may contribute to the observed reduction of kinetochore-localized KNL-1 in embryos depleted of CeNDC-80 or HIM-10 (Fig. 5B)."
"full-length UBXN-3 co-purified with CDC-48 and CDT-1(Fig. 5b,c), thus confirming the in vivo results shown in Fig. 4. In contrast, a truncated version lacking the UBX and CC domain did not interact with CDC-48, however, was still able to bind CDT-1 and ubiquitin conjugates (Fig. 5b). Conversely, a fragment only consisting of the UBX and CC domain exclusively interacted with CDC-48, indicating that CDC-48 alone is not sufficient for CDT-1 binding (Fig. 5b). Remarkably, point mutations of the FPK motif in the UBX domain were completely impaired in CDC-48 interaction; however, fully functional in CDT-1 and ubiquitin-conjugate binding (Fig. 5b; Supplementary Fig. 5a)"
"full-length UBXN-3 co-purified with CDC-48 and CDT-1(Fig. 5b,c), thus confirming the in vivo results shown in Fig. 4. In contrast, a truncated version lacking the UBX and CC domain did not interact with CDC-48, however, was still able to bind CDT-1 and ubiquitin conjugates (Fig. 5b). Conversely, a fragment only consisting of the UBX and CC domain exclusively interacted with CDC-48, indicating that CDC-48 alone is not sufficient for CDT-1 binding (Fig. 5b). Remarkably, point mutations of the FPK motif in the UBX domain were completely impaired in CDC-48 interaction; however, fully functional in CDT-1 and ubiquitin-conjugate binding (Fig. 5b; Supplementary Fig. 5a)"
"KNL-1 still localized to kinetochores in embryos depleted of either ceNDC-80 or HIM-10 (Fig. 5B). However, the level of kinetochore-localized KNL-1 was significantly reduced relative to identically processed control embryos (Fig. 5B)... KNL-1 was reduced to ~50% of wild-type levels in depletions of either CeNDC-80 or HIM-10, suggesting that reduction of KNL-1 protein levels may contribute to the observed reduction of kinetochore-localized KNL-1 in embryos depleted of CeNDC-80 or HIM-10 (Fig. 5B)."
"we cultured blastomeres from ama-1(RNAi)/pes-10::GFP embryos in actinomycin D. Detectable GFP expression was absent, but in all cases EMS mitotic spindle orientation was normal (Fig. 5B)."