"we carried out transgenerational analysis of
unc-119::GFP germline expression at 25C. In early generations (F1 or F2, Table 1), when
set-2(lf) animals are still fertile, most germlines did not express GFP (Figure S2D). This number strongly increased in the F3 generation, at the onset of sterility. Similar result were obtained in wdr- 5.1(lf) animals, but not
set-2(
ok952) animals, in which H3K4 methyltransferase activity is mostly intact (Xiao et al., 2011) and which do not show a Mrt phenotype. In addition, quantitative RT-PCR (qRT-PCR) analysis on dissected gonads showed that, for a set of somatic genes encoding homeodomain transcription factors involved in terminal differentiation of neuronal cell fate (
ceh-2,
ceh-20, and
ceh-43; Aspock et al., 2003; Doitsidou et al., 2013), germline misexpression increased over generations at 25C (Figure 2E). This increase was not observed with other genes such as
haf-9, associated with intestinal differentiation, showing that it is not a general phenomenon."