In wild-type animals, after 2 to 3 hours, the
lag-2::GFP signal increased in the DTCs (Z1.aa/Z4.pp) and decreased in their sister cells (Z1.ap/Z4.pa). After about 5 hours, when the DTCs started to migrate,
lag-2::GFP was expressed nearly exclusively in the DTCs and not in their sisters. In contrast, in 3/8
cye-1 mutants, the GFP signal increased in both daughters of Z1.a or Z4.p, 3 hours after they were born. After 5 hours, both daughter cells started to migrate like wild-type DTCs. They continued to migrate without further cell divisions for at least 8 hours after they were born.