"We observe the greatest degree of regulation, however, amongst the 16 regulators of cytoprotective gene expression that regulate 2 or more of the tested cytoprotective pathways.
lin-40 gene inactivation disrupts
psod-3::gfp induction 17-fold, and
pgst-4::gfp 2-fold.
let-92 gene inactivation results in the most potent disruption of
pgst-4::gfp induction, decreasing expression 25-fold, and inhibiting induction of
psod-3::gfp and
phsp-6::gfp by 3- and 4-fold, respectively.
ima-3 and
elt-2 gene inactivations both dramatically decrease induction of
phsp-6::gfp by antimycin.
ima-3 gene inactivation additionally inhibits the induction of
pgst-4::gfp (4-fold).
elt-2, like
let-70 and
cpsf-2, is required for the appropriate regulation of all four tested cytoprotective pathways (Table 1, Figure 1)... Induction of
phsp-16.2::gfp following heat shock and the expression of a non-stress-induced fusion gene,
psur-5::gfp, were quantified to control for generic transgene silencing phenotypes; none of these gene inactivations were potent transgene silencers (Table S4)"