The expression pattern of a PCR.YB5 transgene (csEx22) was examined in
eff-1;
lin-39(
n1490ts) mutants. Because it has been observed that PCR.YB5 expression decline in cells that fused with
hyp7, the fusogenic mutation
eff-1 was included to prevent Pn.p fusion in this strain. At the mid-L3 stage, 61% of csEx22 animals (n = 18) and 75% of
eff-1;
lin-39ts; csEx22 animals (n = 12) had bright egl-18aDGFP expression in at least one Pn.p cell. These results indicate that egl-18aDGFP is expressed independently of
lin-39 activity.