"worms with mutations causing proliferative tumors (
mog-6(
q465);
gld-3(
q730) (Belfiore et al., 2004) and glp- 1
(ar202) gain of function (Pepper et al., 2003) mutations, respectively) were grown to old age and ectopic expression of MYO-3 was assessed by immunostaining (Figure S5B). Although the tumorous germlines of both mutants were filled with proliferative germ cells, there was no P granule loss or ectopic MYO-3 expression, confirming that muscular differentiation was not simply an age-related process but was specific to the
spr-5;
let-418 tumorous germlines (Figures S5A and S5B)."