"we looked at the distribution of LRP-1 at the apical surface of
hyp7 in
dpy-5(
e61);
let-512(
h510) double mutant animals. The
dpy-5(
e61) phenotype permitted the identification of homozygous
let-512(
h510) segregants prior to L3/L4 arrest. As control we used
dpy-5(
e61) single mutant animals, which had an LRP-1 expression pattern corresponding to wild-type animals (Figure 5A). During the developmental arrest of
dpy-5(
e61);
let-512(
h510) homozygous animals, LRP-1 gradually became less abundant and less regularly distributed at the apical surface of
hyp7 than in control animals that were wild type for
let-512. Furthermore, the endocytic vesicles changed their form and adopted a more tubular or mesh-like appearance (Figure 5B and C). In arrested
dpy-5(
e61);
let-512(
h510) worms, the apical LRP-1 signals disappeared almost completely, although some faint and diffuse LRP-1 signal remained in the cytoplasm of
hyp7 (Figure 5D)."